Key Lab of Industrial Biocatalysis Ministry of Education
人员 研究人员 人员
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XINHUI XING  

Professor


  

Tel:+8610  62794771 

Mail:xhxing@tsinghua.edu.cn

 





●  Education & Professional Career

  • Education

South China University of Technology                          Applied Chemistry                  B.S., 1985

Utsunomiya University, Japan                                     Applied Chemistry                   M.S., 1989

Tokyo Institute of Technology, Japan                          Chemical Engineering             Ph.D., 1992

  • Appointments

Prpfessor, Institute of Biopharmaceutical and Health Engineering,

Tsinghua Shenzhen International Graduate School, Shenzhen                  since 6/2019

Professor, Tsinghua Shenzhen International Graduate School                                 since 7/2019

Senior PI, Institute of  Institute of Biomedical Health Technology and                   since 12/2019

Engineering, Shenzhen Bay Laboratory

Professor, Tsinghua University                                                                                since 9/2000

Lecturer and Associate, Yokohama National University, Japan                             3/1998-7/2001

Assistant Professor, Tokyo Institute of Technology, Japan                                   4/1992-2/1998

  • Admistration work

Deputy director, Institute of Biopharmaceutical and Health Engineering,

Tsinghua Shenzhen International Graduate School, Shenzhen                since 12/2019

Vice Chair, Department of Chemical Engineering, Tsinghua University                 3/2009-6/2018

●  Research interest

Fusion protein design, ezyme engineering, biobreeding technology and instrumentation, high throughput technology, organoid mcriofluidic system, health-care product engineering and glycodrug technology.

●  Selected Publications

1.    Zhidan Liu, Buchun Si, Jiaming Li, Jianwei He, Chong Zhang*, Yuan Lu, Yuanhui Zhang and Xin-Hui Xing*, Bioprocess engineering for biohythane production from low-grade waste biomass: technical challenges towards scale up. Current Opinion in Biotechnology, 50, 25-31 (2018)

2.    Lan-Yu Cui, Shan-Shan Wang, Chang-Ge Guan, Wei-Fan Liang, Zheng-Lian Xue, Chong Zhang,* and Xin-Hui Xing*, Breeding of Methanol-Tolerant Methylobacterium extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution. Biotechnology Journal, 1700679 (2018)

3.    Yang Ji, Yuan Lu, Yishu Yan, Xinxin Liu, Nan Su, Chong Zhang, Shengli Bi*, Xin-Hui Xing* Design of Fusion Proteins for Efficient and Soluble Production of Immunogenic Ebola Virus Glycoprotein in Escherichia coli. Biotechnology Journal, 1700627 (2018)

4.    Wenming Zhu, Yang Ji, Yi Wang, Dong He, Yishu Yan, Nan Su, Chong Zhang and Xin-Hui Xing*, Structural characterization and in vitro antioxidant activities of chondroitin sulfate purified from Andrias davidianus cartilage. Carbohydrate Polymers, 196, 398-404 (2018)

5.    Tianmin Wang##, Changge Guan##, Jiahui Guo, Bing Liu, Yinan Wu, Zhen Xie, Chong Zhang*, and Xin-Hui Xing, Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance. Nature Communications, 9, 2475 (2018)

6.    Zhou YK (Zhou, Yikang), Li G (Li, Gang), Dong JK (Dong, Junkai), Xing XH (Xing, Xin-hui), Dai JBA (Dai, Junbiao), Zhang C (Zhang, Chong)*, MiYA, an efficient machine-learning workflow in conjunction with the YeastFab assembly strategy for combinatorial optimization of heterologous metabolic pathways in Saccharomyces cerevisiae. Metabolic Engineering, 47, 294-302 (2018)

7.    Jiahui Guo##, Tianmin Wang##, Changge Guan, Bing Liu, Cheng Luo, Zhen Xie, Chong Zhang*, Xin-Hui Xing, Improved sgRNA design in bacteria via genome-wide activity profiling. Nucleic Acid Research, 46(14), 7052-7069 (2018)

8.    Hou Ji-Lun, Zhang Xiao-Yan, Wang Gui-Xing, Sun Zhao-Hui, Du Wei, Zhao Ya-Xian, Si Fei, Wang Li-Yan, Xing Xin-Hui*, Wang Yu-Fen*, Novel breeding approach for Japanese flounder using atmosphere and room temperature plasma mutagenesis tool. BMC Genomics, 20: 323 (2019)

9.    Chunxuan Ma, Zhenglin Tan, Ying Lin, Shuangyan Han, Xin-Hui Xing, Chong Zhang*, Gel-microdroplet-based high throughput screening for directed evolution of xylanase-producing Pichia pastoris. Journal of Bioscience and Bioengineering, 128(6): 662-668 (2019)

10.  Yi-Fan Xing, Dong He, Yi Wang, Wen Zeng, Chong Zhang, Yuan Lu, Nan Su, Yan-Hua Kong and Xin-Hui Xing*, Chemical Constituents, Biological Functions and Pharmacological Effects for Comprehensive Utilization of Eucommia ulmoides Oliver. Food Science and Human Wellness, 8(2), 177-188 (2019)

11.  Tan, ZL (Tan, Zheng Lin); Zheng, X (Zheng, Xiang); Wu, YA (Wu, Yinan); Jian, XJ (Jian, Xingjin); Xing, XH (Xing, Xinhui); Zhang, C (Zhang, Chong)*, In vivo continuous evolution of metabolic pathways for chemical production. Microbial Cell Factory, 18: 82 (2019)

12.  Huimin Zhang; Yajing Meng; Yanli Wang; Qingwu Zhou; Anjun Li; Guoying Liu; Jingxin Li; Xin-Hui Xing*, Prokaryotic Communities in Multidimensional Bottom-Pit-Mud from Old and Young Pits Used for the Production of Chinese Strong-Flavor Baijiu. Food Chemistry, 312: 126084 (2020)

13.  Dong He, Yi Wang, Jun Lin, Yi-Fan Xing, Wen Zeng, Wen-Ming Zhu, Nan Su, Chong Zhang, Yuan Lu, and Xin-Hui Xing*, Identification and characterization of alcohol-soluble components from wheat germ-apple fermented by Lactobacillus sp. capable of preventing ulcerative colitis of dextran sodium sulfate-induced mice. Journal of Functional Foods, 64, 103642 (2020)

14.  Wang, TM (Wang, Tianmin)#,* ; Zheng, X (Zheng, Xiang)# ; Ji, HN (Ji, Haonan); Wang, TL (Wang, Ting-Liang); Xing, XH (Xing, Xin-Hui); Zhang, C (Zhang, Chong)*, Dynamics of transcription-translation coordination tune bacterial indole signaling. Nature Chemical Biology, 16, 440-449 (2020)

15.  Yang Ji#, Yi Wang#, Wen Zeng, Xiang Mei, Shanshan Du, Yishu Yan, Jie Hao, Zhenqing Zhang, Yuan Lu, Chong Zhang, Jun Ge and Xin-Hui Xing*. A heparin derivatives library constructed by chemical modification and enzymatic depolymerization for exploitation of non-anticoagulant functions. Carbohydrate Polymers, 249, 116824 (2020)

16.  Liyan Wang# Hongxin Zhao#, Dong He, Yinan Wu, Lihua Jin, Guo Li, Nan Su, Heping Li* and Xin-Hui Xing*, Insights into the molecular-level effects of atmospheric and room-temperature plasma on mononucleotides and single-stranded homo- and hetero-oligonucleotides. Scientific Reports, 10:14298  (2020)

17.  Xingjin Jian#, Xiaojie Guo#, Jia Wang, ZhengLin Tan, Xin–Hui Xing, Liyan Wang* and Chong Zhang*, Microbial microdroplet culture system (MMC): an integrated platform for automated, high–throughput microbial cultivation and adaptive evolution. Biotechnology and Bioengineering, 117 (6), 1724-1737 (2020)

18.  Dong He#, Xue Peng#, Yi-Fan Xing, Yi Wang, Wen Zeng, Nan Su, Chong Zhang, Dian-Nan Lu*, Xin-Hui Xing*, Increased stability and intracellular antioxidant activity of chlorogenic acid depend on its molecular interaction with wheat gluten hydrolysate. Food Chemistry, 325:12687 (2020)

19.  Li, JM (Li, Jiaming); He, JW (He, Jianwei); Si, BC (Si, Buchun) ; Liu, ZD (Liu, Zhidan) *; Zhang, C (Zhang, Chong)*; Wang, YX (Wang, Yuxin); Xing, XH (Xing, Xin-hui)*, A pilot study of biohythane production from cornstalk via two-stage anaerobic fermentation. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,45 (56),31719-31731 (2020)

20.  Jia Wang, Xinjing Jian,  Xin-Hui Xing,  Chong Zhang*, Qiang Fei*, Empowering a Methanol-Dependent Escherichia coli via Adaptive Evolution Using a High-Throughput Microbial Microdroplet Culture System. Frontiers in Bioengineering and Biotechnology, 8: 570 (2020)

21.  Jun-Yu Liu, Dong He, Yi-Fan Xing, Wen Zeng, Kai Ren, Chong Zhang, Yuan Lu, Song Yang, Shu-Jian Ou, Yi Wang *, Xin-Hui Xing*, Effects of bioactive components of Pu-erh tea on gut microbiomes and health: A review. Food Chemistry, 353, 129439 (2021)

22.  Yifan XING, Junyu LIU, Xiaojie GUO, Haipeng LIU, Wen ZENG, Yi WANG, Chong ZHANG, Yuan LU, Dong HE, Shaohua Ma, Yonghong He, Xin-Hui XING*, Engineering organoid microfluidic system for biomedicine and health engineering: a review. Chinese Journal of Chemical Engineering, 30, 244-254 (2021)

23.  Xiao-Jie Yuan, Wen-Jing Chen, Zeng-Xin Ma, Qian-Qian Yuan, Min Zhang, Lian He, Xu-Hua Mo, Chong Zhang, Chang-Tai Zhang, Meng-Ying Wang, Xin-Hui Xing and Song Yang* Rewiring the native methanol assimilation metabolism by incorporating the heterologous ribulose monophosphate cycle into Methylorubrum extorquens. Metabolic Engineering, 64,  95-110 (2021)

24.  Yuting Li#, Dong He#, Bing Li, Marianne N. Lund, YifanXing, Yi Wang, Fuxiang Li, Xiao Cao, Yujia Liu, Xiangyu Chen, Jiamei Yu, Jie Zhu, Minlian Zhang, Qiang Wang, Yuhao Zhang, Bin Li, Jinshui Wang, Xin-Hui Xing* and Lin Li*, Engineering polyphenols with biological functions via polyphenol-protein interactions as additives for functional foods. Trends in Food Science & Technology, 110, 470–482 (2021)

25.  Yuting Huang#, Liyang Wang#, Xue Zhang, Nan Su, Heping Li, Yoshimitsu Oda, Xinhui Xing*, Quantitative evaluation of DNA damage caused by Atmospheric and Room-Temperature Plasma (ARTP) and other mutagenesis methods using a rapid umu-microplate test protocol for microbial mutation breeding. Journal of Chemical Engineering, in press (2021)

26.  Wen Zeng, Dong He, Yifan Xing, Junyu Liu, Nan Su, Chong Zhang, Yi Wang*, Xinhui Xing*, Internal connections between dietary intake and gut microbiota homeostasis in disease progression of ulcerative colitis: a review. Food Science and Human Wellness, 10, 119-130 (2021)

27.  Zeng-xin Ma, Min Zhang, Chang-tai Zhang, Hui Zhang, Xu-hua Mo, Xin-hui Xing, Song Yang*, Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM1. Biotechnology Journal, e2000413 (2021)

28.  Chenqi Niu, Chuyi Wang, Fan Li, Xiang Zheng, Xinhui Xing, Chong Zhang*, Aptamer assisted CRISPR-Cas12a strategy for small molecule diagnostics. Biosensors and Bioelectronics,  183, 113196 (2021)

29.  Yinan Wu, Aysha Jameel, Xin-Hui Xing and Chong Zhang*, Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution. Trends in Biotechnology,  in press (2021)

30.  Xi Wu*, Chong Zhang, Xin-Hui Xing, Zhenyu Yun, Lin Zhao, Qi Wu, Construction and characterization of novel bifunctional fusion proteins composed of alcohol dehydrogenase and NADH oxidase with efficient oxidized cofactor regeneration. Biotechnology and Applied Biochemistry, 1-10 (2021)

31.  Huibao Feng#, Yaomeng Yuan#, Zheng Yang, Xin-hui Xing, and Chong Zhang*, Genome-wide genotype-phenotype associations in microbes. Journal of Bioscience and Bioengineering, 132 (1), 1-8 (2021)

32.  Huibao Feng#, Jiahui Guo#, Tianmin Wang#*, Chong Zhang* and Xin-hui Xing, Guide-target mismatch effects on dCas9–sgRNA binding activity in living bacterial cells. Nucleic Acids Research, 49 (3), 1263–1277 (2021)

33.  Yi Wu#, Dong He#, Minhua Zong, Hong Wu, Lin Li, Xia Zhang, Xin-Hui Xing* and Bing Li*, Improvement in the stability and bioavailability of trans-resveratrol with hydrolyzed wheat starch complexation: a theoretical and experimental study. Food Structure, 32, 100267 (2022)