报告题目：Biocompatible Polymer Interface for Biodevices

报 告 人：Prof. Madoka Takai The University of Tokyo

报告时间：2019年10月24日 上午10:30-12:00

报告地点：英士楼319会议室

Abstract：

Miniaturized biomedical devices for diagnostics, drug screening have become the promising tools as the next generation clinical use. Many typed microchip-base biodevices are studied and applied for practical, because of achieving the high throughput assay. However, there are still problems to overcome, and one the biggest one is a decrease of non-specific protein adsorption on device surface. Thus, we have developed a functional polymer consisting of 2-methacryloyloxyethyl phosphorylcholine (MPC), which has a biocompatible property to reduce protein adsorption. We designed MPC polymer with cross-linking structure to control stiffness, water content and gas transport in addition of anti-biofouling. This polymer has a cross-linking unit of 3-methacryloxypropyltrimethoxysilane (MPTMSi), and a hydrophobic unit of 3-(methacryloyloxy) propyltris (trimethylsiloxy) silane (MPTSSi). And it could stably coat on silicone elastomer as substrate for up to 12 weeks. The protein adsorption and cell adhesion were reduced on the surface of MPC polymer modification. We also investigated bacterial adhesion, and S. aureus was hardly adsorbed on the MPC coating surface. Another application to use MPC polymer, nanoparticles as cell imaging probe has developed to detect sialic acid which is well known biomarker of disease. The imaging nanoparticle showed good selectivity for target molecule due to the function of MPC. Finally, protein adsorption mechanism on the interface of MPC polymer is summarized to compare polymers which has hydrophobic, hydrophilic, electrostatic and zwitterionic properties.

Selected Publications:

[1] Stable surface coating of silicone elastomer with phosphorylcholineand organosilane copolymer with cross-linking for repelling proteins, K. Nagahashi, Y. Teramura, and M. Takai, Colloids and Surfaces B: Biointerfaces 134, 384–391 (2015).

[2] Lectin-tagged Fluorescent Polymeric Nanoparticles for Targe