长期从事先进生物制造、合成生物学及纳米生物技术研究。主持国家重点研发、973、863、国家自然科学基金等课题30余项，在Biotechnology Advances, Metabolic Engineering等期刊发表论文130余篇，专著章节5部，申请/授权发明专利50余项。产业化已创利税10亿多元。曾获教育部技术发明及自然科学二等奖、中石化工联合会技术发明二等奖、青年科技突出贡献奖等。入选教育部新世纪优秀人才、全国百篇优博论文作者、北京市科技新星（A类）。
● 教育与工作经历
1991-1996 清华大学化学工程系，学士
1996-2001 清华大学化学工程系，博士
2000-2003 清华大学化学工程系，讲师
2005-2006 美国麻省理工学院化学工程系，访问学者
2003-2012 清华大学化学工程系，副教授
2011-2016 清华大学(化工系)-山东宝莫股份 生物化学品联合研究中心 主任
2012-2016，清华大学化学工程系，研究员，博导
2016-至今，清华大学化工系，教研系列长聘教授
2022-至今，工业生物催化教育部重点实验室 副主任
● 研究领域
工业生物催化、合成生物学、微生物代谢工程、生物纳米技术。
● 开设课程
《生物化学（1）》，本科生课程
《分子酶工程》，研究生课程
● 主要学术任职
● 代表性论文
1. Youxiang Liang, Huimin Yu*. Genetic toolkits for engineering Rhodococcus species with versatile applications. Biotechnology Advances, 2021, 49: 107748.
2. Fulong Li, Youxiang Liang, Yuwen Wei, Yukun Zheng, Yan Du and Huimin Yu*. Biochemical and structural characterization of an (R)-selective transaminase in the asymmetric synthesis of chiral hydroxy amines. Advanced Synthesis & Catalysis, 2021, 363, 1–9.
3. Yan Du, Fangyu Cheng, Miaomiao Wang, Chunmeng Xu and Huimin Yu*. Indirect pathway metabolic engineering strategies for enhanced biosynthesis of hyaluronic acid in engineered Corynebacterium glutamicum. Frontiers in Bioengineering and Biotechnology. 2021, 9:768490.
4. Wenjing Qi, Huimin Yu*. Virus-templated magnetic composite hydrogels for surface immobilization of mimic-free-lipase. Nanoscale, 2021, 13, 17871 – 17880.
5. Youxiang Liang#, Yuwen Wei#, Song Jiao and Huimin Yu*. A CRISPR/ Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630. Synthetic and Systems Biotechnology, 2021, 6: 200–208.
6. Youxiang Liang, Song Jiao, Miaomiao Wang, Huimin Yu* and Zhongyao Shen. A CRISPR/Cas9-based genome editing system for Rhodococcus. Metabolic Engineering, 2020, 57: 13-22.
7. Yukun Zheng#, Fangyu Cheng#, Bo Zheng and Huimin Yu*. Enhancing single-cell hyaluronic acid biosynthesis by microbial morphology engineering. Synthetic and Systems Biotechnology, 2020, 5, 316–323.
8. Miaomiao Wang, Huimin Yu*, Xu Li, Zhongyao Shen. Single-gene regulated non-spore-forming Bacillus subtilis: different patterns, transcriptome responses and applications for producing enzymes and surfactin. Metabolic Engineering, 2020. 62：235–248.
9. Chunmeng Xu, Lingjun Tang, Youxiang Liang, Song Jiao, Huimin Yu,* and Hui Luo. Novel chaperones RrGroEL and RrGroES for activity and stability enhancement of nitrilase in Escherichia coli and Rhodococcus ruber. Molecules. 2020, 25(4):1002-1016.
10. Song Jiao, Fulong Li, Huimin Yu* and Zhongyao Shen. Advances in acrylamide bioproduction catalyzed with Rhodococcus cells harboring nitrile hydratase. Applied Microbiology and Biotechnology, 2020, 104(3), 1001-1012.
11. Fangyu Cheng, HuiminYu*, Gregory Stephanopoulos*. Engineering Corynebacterium glutamicum for high-titer biosynthesis of hyaluronic acid. Metabolic Engineering, 2019, 55:276-289.
12. Miaomiao Wang*, Huimin Yu*, Zhongyao Shen. Antisense RNA-based strategy to enhance ACCase activity and surfactin production in Bacillus subtilis TS1726. ACS synthetic biology. 2019, 8: 251-256.
13. Miaomiao Wang#, Wenjing Qi#, Hongping Xu, Huimin Yu*, Shuliang Zhang, Zhongyao Shen. Affinity binding immobilization of D-amino acid oxidase on mesoporous silica by a silica-specific peptide. Journal of Industrial Microbiology & Biotechnology, 2019. 1-7.
14. Lingjun Tang#, Ji Yang#, Jie Chen, Jing Zhang, Huimin Yu* and Zhongyao Shen. Design of salt-bridge cyclization peptide tags for stability and activity enhancement of enzymes. Process Biochemistry 2019, 81: 39-47.
15. Youxiang Liang, Song Jiao, Miaomiao Wang, Huimin Yu* and Zhongyao Shen. Overexpression of epoxide hydrolase in Rhodococcus ruber with high robustness for the synthesis of chiral epichlorohydrin. Process Biochemistry. 2019, 79, 49-56.
16. Song Jiao, Huimin Yu* and Zhongyao Shen. Core elements characterization of Rhodococcus promoters and development of a gradient intensity mini-pool for efficient gene expression. New Biotechnology, 2018, 44, 41-49.
17. Qi Wang, Huimin Yu*, Miaomiao Wang, Huan Yang and Zhongyao Shen. Enhanced biosynthesis and characterization of surfactin isoforms with engineered Bacillus subtilis through promoter Replacement and Vitreoscilla hemoglobin co-expression. Process Biochemistry, 2018, 70，36-44.
18. Miaomiao Wang*, Jie Chen, Huimin Yu*, Zhongyao Shen. Improving stress tolerance and cell integrity of Rhodococcus ruber by overexpressing small‑shock‑protein Hsp16 of Rhodococcus. Journal of Industrial Microbiology & Biotechnology (2018) 45:929–938.
19. Yangzi Chen, Song Jiao, Miaomiao Wang, Jie Chen, Huimin Yu*. A novel molecular chaperone GroEL2 from Rhodococcus ruber and its fusion chimera with nitrile hydratase for co-enhanced activity and stability. Chemical Engineering Science, 192 (2018) 235–243.
20. Fangyu Cheng, Sijin Luozhong, Zhigang Guo, Huimin Yu* and Gregory Stephanopoulos. Enhanced biosynthesis of hyaluronic acid with engineered Corynebacterium glutamicum via metabolic pathway regulation. Biotechnology Journal. 2017, 12, 1700191.
21. Ji Yang, Fangyu Cheng, Huimin Yu*, Junting Wang, Zhigang Guo and Gregory Stephanopoulos. Key role of the carboxyl terminus of hyaluronan synthase in processive synthesis and size control of hyaluronic acid polymers. Biomacromolecules 2017, 18, 1064−1073.
22. Song Jiao, Jie Chen, Huimin Yu*, Zhongyao Shen. Tuning and elucidation of the colony dimorphism in Rhodococcus ruber associated with cell flocculation in large scale fermentation. Appl Microbiol Biotechnol., 2017, 101:6321-6332.
23. Song Jiao, Xu Li, Huimin Yu*, Huan Yang, Xue Li and Zhongyao Shen. In situ enhancement of surfactin biosynthesis in Bacillus subtilis using novel artificial inducible promoters. Biotechnology and Bioengineering 2017, 114(4): 832–842.
24. Shuai Zhang, Huimin Yu*, Ji Yang, Zhongyao Shen. Design of the nanoarray-pattern Fe-Ni bi-metal nanoparticles@M13 virus for enhanced reduction of p-chloronitrobenzene through micro-electrolysis effect. Environmental Science: Nano, 2017, 4, 876 - 885.
25. Jizhe Sun, Huimin Yu*, Jie Chen, Hui Luo and Zhongyao Shen. Ammonium acrylate biomanufacturing by an engineered Rhodococcus ruber with nitrilase overexpression and double-knockout of nitrile hydratase and amidase. Journal of Industrial Microbiology & Biotechnology. 2016, 43(12):1631-1639.
26. Huan Yang, Xu Li, Xue Li, Huimin Yu* and Zhongyao Shen. Identification of Lipopeptide Isoforms by Tandem MALDI-TOF-MS/MS Based on the Simultaneous Purification of Iturin, Fengycin and Surfactin by RP-HPLC. Analytical and Bioanalytical Chemistry. 2015, 407(9): 2529-2542.
27. Xu Li, Huan Yang, Donglai Zhang, Xue Li, Huimin Yu* & Zhongyao Shen. Overexpression of specific proton motive force-dependent transporters facilitate the export of surfactin in Bacillus subtilis. Journal of Industrial Microbiology & Biotechnology: 2015, 42(1), 93-103.
28. Jie Chen, Huimin Yu*, Changchun Liu, Jie Liu and Zhongyao Shen. Improving stability of nitrile hydratase by bridging the salt-bridges in specific thermal-sensitive regions. Journal of Biotechnology, 2013, 164: 354–362.
29. Huimin Yu,* Keith Tyo, H. Alper, D. Marcuschamer, Gregory Stephanopoulos*. A High Throughput Screen for Hyaluronic Acid Accumulation in Recombinant Escherichia coli Transformed by Libraries of Engineered Sigma Factors. Biotechnology and Bioengineering. 2008, 101 (4): 788-796.
30. Huimin Yu* and Gregory Stephanopoulos*. Metabolic Engineering of Escherichia coli for biosynthesis of hyaluronic acid. Metabolic Engineering. 2008, 10(1): 24-32.
● 授权专利
1. 2022，一种重组转氨酶和所述重组转氨酶的突变体及其应用，专利号：ZL202011280191.3。
2. 2021，一种高产脂肽的基因工程菌及其应用，专利号：ZL201811465067.7。
3. 2021，一种生产软骨素的基因工程菌及其构建方法和应用。专利号：201811277262.7。
4. 2021，一种基因工程红球菌及其构建方法与应用。专利号：201910880078.X。
5. 2021，一种高产脂肽的工程菌构建方法及其应用。专利号：201810865295.7。
6. 2021，一种表达环氧化物水解酶的工程菌及其构建方法和应用。专利号：201811104611.5。
7. 2021，一种高产透明质酸的重组菌及其构建方法与应用。专利号：ZL 201910495504.8
8. 2021，一种高产脂肽的重组菌及其应用。专利号：ZL 201910549289.5。
9. 2020，一种改造腈水合酶及其应用。专利号：ZL 201710456875.6。
10. 2020，双基因敲除重组红球菌、构建方法及其应用。专利号：ZL 201510946072.X。
11. 2018，一种枯草芽孢杆菌诱导性强启动子及其应用. 专利号：ZL 201510654218.3。
12. 2018，一种钩状结构寡肽及其应用. 专利号：ZL 201510149607.0。
13. 2018，微电解体系及其制备方法和应用. 专利号：ZL 201610109318.2。
14. 2016，一种高表达脂肽类生物表面活性剂的工程菌及其应用. 专利号：ZL 20140124438.0。
15. 2016，一种生产透明质酸的基因工程菌及其应用. 专利号：ZL 20140166015.5。
16. 2016，一种M13噬菌体介导的纳米铁还原镉离子的方法. 专利号：ZL 200410090776.7。
17. 2014，一种突变头孢菌素C酰化酶. 专利号：ZL 2012 1 04669788。
18. 2013，An engineered nitrile hydradase-producing bacterium with amidase gene knocked-out, the construction and the use thereof. 美国发明专利号：US 8518685。
19. 2013，一种突变腈水合酶. 专利号：ZL 201110415465.X。
20. 2012，一种高丙烯酰胺和丙烯腈耐受性产腈水合酶工程菌及应用. 专利号：ZL201010188495.7。
● 学术荣誉与奖励
2017年，高等学校科学研究优秀成果奖技术发明二等奖（第一完成人），教育部
2016年，东营市科学技术合作奖，东营市科技局
2013年, 技术发明二等奖（第1完成人），中国石油和化学工业联合会
2010年, 青年科技突出贡献奖，中国石油和化学工业联合会
2008年, 新世纪优秀人才，教育部
2007年, 北京市科技新星（A类)，北京市科学技术委员会
2005年, 华新杰出学者，清华大学
2003年, 全国百篇优秀博士学位论文奖 ，国务院和教育部
2003年, 教育部提名国家科学技术奖自然科学奖二等奖（第2完成人）
● 实验室主页
http://cbc.chemeng.tsinghua.edu.cn:8080